study caki 1 Search Results


study caki 1  (ATCC)
99
ATCC study caki 1
Study Caki 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caki 1  (ATCC)
97
ATCC caki 1
Caki 1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human caki-1 rcc cells (htb-46)  (Charles River Laboratories)
90
Charles River Laboratories human caki-1 rcc cells (htb-46)
Human Caki 1 Rcc Cells (Htb 46), supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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study a 498  (ATCC)
96
ATCC study a 498
Study A 498, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ccrcc cell lines  (ATCC)
98
ATCC human ccrcc cell lines
KMT5C expression is upregulated in several <t>ccRCC</t> cell lines. (A) The protein expression levels of KMT5C in four ccRCC cell <t>lines</t> <t>(786O,</t> <t>A498,</t> Caki-1 and OSRC2), as well as HK2 human proximal tubular epithelial cells, were examined by western blotting. (B) The mRNA expression levels of KMT5C in four ccRCC cell lines (786O, A498, Caki-1 and OSRC2), as well as HK2 human proximal tubular epithelial cells, were analyzed by RT-qPCR (n=3). The KMT5C mRNA expression levels in (C) A498 and (D) OSRC2 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were analyzed by RT-qPCR (n=3). (E) The protein expression levels of KMT5C in A498 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were assessed by western blotting and (F) compared statistically. (G) The protein expression levels of KMT5C in OSRC2 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were assessed by western blotting and (H) compared statistically. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 using one-way ANOVA and Dunnett's test. ccRCC, clear cell renal cell carcinoma; KMT5C, lysine methyltransferase 5C; ns, not significant; sh, short hairpin.
Human Ccrcc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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KMT5C expression is upregulated in several ccRCC cell lines. (A) The protein expression levels of KMT5C in four ccRCC cell lines (786O, A498, Caki-1 and OSRC2), as well as HK2 human proximal tubular epithelial cells, were examined by western blotting. (B) The mRNA expression levels of KMT5C in four ccRCC cell lines (786O, A498, Caki-1 and OSRC2), as well as HK2 human proximal tubular epithelial cells, were analyzed by RT-qPCR (n=3). The KMT5C mRNA expression levels in (C) A498 and (D) OSRC2 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were analyzed by RT-qPCR (n=3). (E) The protein expression levels of KMT5C in A498 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were assessed by western blotting and (F) compared statistically. (G) The protein expression levels of KMT5C in OSRC2 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were assessed by western blotting and (H) compared statistically. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 using one-way ANOVA and Dunnett's test. ccRCC, clear cell renal cell carcinoma; KMT5C, lysine methyltransferase 5C; ns, not significant; sh, short hairpin.

Journal: International Journal of Oncology

Article Title: Lysine methyltransferase 5C increases the proliferation and metastatic abilities of clear cell renal cell carcinoma via aerobic glycolysis

doi: 10.3892/ijo.2024.5633

Figure Lengend Snippet: KMT5C expression is upregulated in several ccRCC cell lines. (A) The protein expression levels of KMT5C in four ccRCC cell lines (786O, A498, Caki-1 and OSRC2), as well as HK2 human proximal tubular epithelial cells, were examined by western blotting. (B) The mRNA expression levels of KMT5C in four ccRCC cell lines (786O, A498, Caki-1 and OSRC2), as well as HK2 human proximal tubular epithelial cells, were analyzed by RT-qPCR (n=3). The KMT5C mRNA expression levels in (C) A498 and (D) OSRC2 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were analyzed by RT-qPCR (n=3). (E) The protein expression levels of KMT5C in A498 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were assessed by western blotting and (F) compared statistically. (G) The protein expression levels of KMT5C in OSRC2 cells stably expressing knockdown control, shKMT5C-1, shKMT5C-2 and shKMT5C-3 plasmids were assessed by western blotting and (H) compared statistically. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 using one-way ANOVA and Dunnett's test. ccRCC, clear cell renal cell carcinoma; KMT5C, lysine methyltransferase 5C; ns, not significant; sh, short hairpin.

Article Snippet: In the present study, three human ccRCC cell lines (786O, A498 and Caki-1), 293T cells and a human proximal tubular epithelial cell line, HK2, were obtained from the American Type Culture Collection.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Stable Transfection, Knockdown, Control